PALF Acts as Both a Single-Stranded DNA Endonuclease and a Single-Stranded DNA 3’-Exonuclease and Can Participate in DNA End Joining in a Biochemical System
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چکیده
Background: PALF binds to the NHEJ protein, XRCC4. Results: PALF is a single-stranded DNA endonuclease and 3’ exonuclease. PALF can coordinate with known NHEJ proteins to achieve ligation. Conclusion: In vitro and in vivo, PALF is able to cooperate with NHEJ proteins to join double-stranded DNA breaks. Significance: A second nuclease, in addition to Artemis, can function with NHEJ proteins to achieve DNA end joining.
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تاریخ انتشار 2011